diff --git a/libavformat/mxfenc.c b/libavformat/mxfenc.c
index 722830615e..f2be76cc86 100644
--- a/libavformat/mxfenc.c
+++ b/libavformat/mxfenc.c
@@ -500,6 +500,7 @@ static const MXFLocalTagPair mxf_local_tag_batch[] = {
     { 0x3302, {0x06,0x0E,0x2B,0x34,0x01,0x01,0x01,0x01,0x04,0x01,0x05,0x01,0x05,0x00,0x00,0x00}}, /* Horizontal Subsampling */
     { 0x3308, {0x06,0x0E,0x2B,0x34,0x01,0x01,0x01,0x02,0x04,0x01,0x05,0x01,0x10,0x00,0x00,0x00}}, /* Vertical Subsampling */
     { 0x3303, {0x06,0x0E,0x2B,0x34,0x01,0x01,0x01,0x01,0x04,0x01,0x05,0x01,0x06,0x00,0x00,0x00}}, /* Color Siting */
+    { 0x3307, {0x06,0x0E,0x2B,0x34,0x01,0x01,0x01,0x02,0x04,0x18,0x01,0x04,0x00,0x00,0x00,0x00}}, /* Padding Bits */
     { 0x3304, {0x06,0x0E,0x2B,0x34,0x01,0x01,0x01,0x01,0x04,0x01,0x05,0x03,0x03,0x00,0x00,0x00}}, /* Black Ref level */
     { 0x3305, {0x06,0x0E,0x2B,0x34,0x01,0x01,0x01,0x01,0x04,0x01,0x05,0x03,0x04,0x00,0x00,0x00}}, /* White Ref level */
     { 0x3306, {0x06,0x0E,0x2B,0x34,0x01,0x01,0x01,0x02,0x04,0x01,0x05,0x03,0x05,0x00,0x00,0x00}}, /* Color Range */
@@ -1147,7 +1148,7 @@ static void mxf_write_cdci_common(AVFormatContext *s, AVStream *st, const UID ke
     int stored_height = (st->codecpar->height+15)/16*16;
     int display_height;
     int f1, f2;
-    unsigned desc_size = size+8+8+8+8+8+8+8+5+16+4+12+20+5 + 5*8;
+    unsigned desc_size = size+8+8+8+8+8+8+8+5+16+4+12+20+5 + 5*8 + 6;
     if (sc->interlaced && sc->field_dominance)
         desc_size += 5;
     if (sc->signal_standard)
@@ -1228,6 +1229,10 @@ static void mxf_write_cdci_common(AVFormatContext *s, AVStream *st, const UID ke
     mxf_write_local_tag(pb, 1, 0x3303);
     avio_w8(pb, sc->color_siting);
 
+    // Padding Bits
+    mxf_write_local_tag(pb, 2, 0x3307);
+    avio_wb16(pb, 0);
+
     if (st->codecpar->color_range != AVCOL_RANGE_UNSPECIFIED) {
         int black = 0,
             white = (1<<sc->component_depth) - 1,
diff --git a/tests/ref/fate/copy-trac4914 b/tests/ref/fate/copy-trac4914
index f868753d45..e3266abeb9 100644
--- a/tests/ref/fate/copy-trac4914
+++ b/tests/ref/fate/copy-trac4914
@@ -1,4 +1,4 @@
-2296e01ba6794ab91c78d8bc1215a801 *tests/data/fate/copy-trac4914.mxf
+298afac2f4ddfed331294981160023f0 *tests/data/fate/copy-trac4914.mxf
 561721 tests/data/fate/copy-trac4914.mxf
 #tb 0: 1001/30000
 #media_type 0: video
diff --git a/tests/ref/fate/mxf-reel_name b/tests/ref/fate/mxf-reel_name
index 50023ce52a..b9beffef66 100644
--- a/tests/ref/fate/mxf-reel_name
+++ b/tests/ref/fate/mxf-reel_name
@@ -1 +1 @@
-c5752bf1b72694455e1b348cad8660c5
+471e8a7d012fb2b00835aadd5fe648c7
diff --git a/tests/ref/fate/time_base b/tests/ref/fate/time_base
index 979aa08972..acda7c5fcf 100644
--- a/tests/ref/fate/time_base
+++ b/tests/ref/fate/time_base
@@ -1 +1 @@
-6aac6c1a2e367e43d665bdb974bb7679
+68608a7f54b8fe3a85cd387e64bab764
diff --git a/tests/ref/lavf/mxf b/tests/ref/lavf/mxf
index df1c2607c7..9f5818e0af 100644
--- a/tests/ref/lavf/mxf
+++ b/tests/ref/lavf/mxf
@@ -1,9 +1,9 @@
-b2c881236ca2791a6f378545d82a891b *./tests/data/lavf/lavf.mxf
+408b3758c11af3210c722a52d2754d8c *./tests/data/lavf/lavf.mxf
 526393 ./tests/data/lavf/lavf.mxf
 ./tests/data/lavf/lavf.mxf CRC=0x8dddfaab
-b14305ee249cc9a7f1708737dbd6daa7 *./tests/data/lavf/lavf.mxf
+76d3bec8f170e389da50a82075c8bf83 *./tests/data/lavf/lavf.mxf
 561721 ./tests/data/lavf/lavf.mxf
 ./tests/data/lavf/lavf.mxf CRC=0xf21b1b48
-0c8ddc85308c8e00d588c33f2458d4be *./tests/data/lavf/lavf.mxf
+caa15603349cb79d11b75a7c6ad633ab *./tests/data/lavf/lavf.mxf
 526393 ./tests/data/lavf/lavf.mxf
 ./tests/data/lavf/lavf.mxf CRC=0x8dddfaab
diff --git a/tests/ref/lavf/mxf_d10 b/tests/ref/lavf/mxf_d10
index 3f91f9c30e..e73cd5d24f 100644
--- a/tests/ref/lavf/mxf_d10
+++ b/tests/ref/lavf/mxf_d10
@@ -1,3 +1,3 @@
-e167b53d927a43d6b14c5d0631640adb *./tests/data/lavf/lavf.mxf_d10
+eb9412edd406c02e4d2ac2380748186b *./tests/data/lavf/lavf.mxf_d10
 5332013 ./tests/data/lavf/lavf.mxf_d10
 ./tests/data/lavf/lavf.mxf_d10 CRC=0x6c74d488
diff --git a/tests/ref/lavf/mxf_dv25 b/tests/ref/lavf/mxf_dv25
index 992f0a927d..8635484f17 100644
--- a/tests/ref/lavf/mxf_dv25
+++ b/tests/ref/lavf/mxf_dv25
@@ -1,3 +1,3 @@
-7ff3cc1ee0a68cc08221c28a1b801490 *./tests/data/lavf/lavf.mxf_dv25
+eddb23aba03c1c0499928bca27d43795 *./tests/data/lavf/lavf.mxf_dv25
 3834413 ./tests/data/lavf/lavf.mxf_dv25
 ./tests/data/lavf/lavf.mxf_dv25 CRC=0xbdaf7f52
diff --git a/tests/ref/lavf/mxf_dvcpro50 b/tests/ref/lavf/mxf_dvcpro50
index 74b0bd17de..5df967054c 100644
--- a/tests/ref/lavf/mxf_dvcpro50
+++ b/tests/ref/lavf/mxf_dvcpro50
@@ -1,3 +1,3 @@
-b6b24c4c562e2d4c866a3f53907c8745 *./tests/data/lavf/lavf.mxf_dvcpro50
+cfbd162ecd9fa35f9f81d0b721f965e3 *./tests/data/lavf/lavf.mxf_dvcpro50
 7431213 ./tests/data/lavf/lavf.mxf_dvcpro50
 ./tests/data/lavf/lavf.mxf_dvcpro50 CRC=0xe3bbe4b4
diff --git a/tests/ref/lavf/mxf_opatom b/tests/ref/lavf/mxf_opatom
index c72481b193..57829f0ebd 100644
--- a/tests/ref/lavf/mxf_opatom
+++ b/tests/ref/lavf/mxf_opatom
@@ -1,3 +1,3 @@
-6cf21f1d5e2406c50edf9c34ecdf2b45 *./tests/data/lavf/lavf.mxf_opatom
+062eb7acb97409a91791ca11374a4b5d *./tests/data/lavf/lavf.mxf_opatom
 4717625 ./tests/data/lavf/lavf.mxf_opatom
 ./tests/data/lavf/lavf.mxf_opatom CRC=0xf55aa22a
diff --git a/tests/ref/lavf/mxf_opatom_audio b/tests/ref/lavf/mxf_opatom_audio
index f020530cc7..db90cf2718 100644
--- a/tests/ref/lavf/mxf_opatom_audio
+++ b/tests/ref/lavf/mxf_opatom_audio
@@ -1,3 +1,3 @@
-0778fda8cea977d37674868ad8ddc2fd *./tests/data/lavf/lavf.mxf_opatom_audio
+b05d38be5e3e40bfe9a9de775370a7ed *./tests/data/lavf/lavf.mxf_opatom_audio
 102969 ./tests/data/lavf/lavf.mxf_opatom_audio
 ./tests/data/lavf/lavf.mxf_opatom_audio CRC=0xd155c6ff
